Heparan sulfate-protein binding specificity
نویسندگان
چکیده
منابع مشابه
Use of sulfated linked cyclitols as heparan sulfate mimetics to probe the heparin/heparan sulfate binding specificity of proteins.
Heparin and heparan sulfate (HS) are structurally diverse glycosaminoglycans (GAG) that are known to interact, via unique structural motifs, with a wide range of functionally distinct proteins and modulate their biological activity. To define the GAG motifs that interact with proteins, we assessed the ability of 15 totally synthetic HS mimetics to interact with 10 functionally diverse proteins ...
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Commercially available systems provide a variety of strategies for the expression and purification of recombinant proteins. One popular method is the polyhistidine fusion tag, which usually consists of six consecutive histidines (6×His) added to the amino or carboxyl terminus of a protein (7). The high-affinity binding of 6×His to metalchelate resins allows easy purification of 6×His-tagged pro...
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Neural circuits consist of distinct neuronal cell types connected in specific patterns. The specificity of these connections is achieved in a series of sequential developmental steps that involve the targeting of neurites, the identification of synaptic partners, and the formation of specific types of synapses. Cell-surface proteins play a critical role in each of these steps. The heparan sulfa...
متن کاملInteractions between heparan sulfate and proteins: the concept of specificity
Proteoglycan (PG) coreceptors carry heparan sulfate (HS) chains that mediate interactions with growth factors, morphogens, and receptors. Thus, PGs modulate fundamental processes such as cell survival, division, adhesion, migration, and differentiation. This review summarizes recent biochemical and genetic information that sheds new light on the nature of HS-protein binding. Unexpectedly, many ...
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BACKGROUND The antithrombin-heparin/heparan sulfate (H/HS) and thrombin-H/HS interactions are recognized as prototypic specific and non-specific glycosaminoglycan (GAG)-protein interactions, respectively. The fundamental structural basis for the origin of specificity, or lack thereof, in these interactions remains unclear. The availability of multiple co-crystal structures facilitates a structu...
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ژورنال
عنوان ژورنال: Biochemistry (Moscow)
سال: 2013
ISSN: 0006-2979,1608-3040
DOI: 10.1134/s0006297913070055